Fitc solubility
WebFITC is tested for solubility and solution appearance at 1 mg/ml in acetone. It is soluble in anhydrous dimethyl sulfoxide (DMSO) at 5 mg/ml.10 It is soluble in water at less than … WebFITC-dextrans have from 0.001-0.020 mol FITC per glucose unit. These low levels of substitution confer minimal charges to dextran, which is an essential requirement for permeability studies. Spectral data FITC-dextran has an excitation maximum of 493 nm and an emission maximum of 518 nm. Maximal intensity is observed at pH >8. Storage and …
Fitc solubility
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WebFITC-Dextran (60842-46-8 MW 4000) is a marker composed of fluorescein isothiocyanate conjugated to 4 kDa dextran, widely used in microcirculation and cell permeability studies. 别名 荧光素异硫氰酸酯-葡聚糖, FITC-Dextran (60842-46-8 MW 4000) WebFITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu 2+ -sensitive fluorescence dye [1] [2] . In Vitro. Annexin V-FITC/PI is employed to detect chondrocyte …
WebOct 13, 2024 · The lesser solubility is also demonstrated by the creation of 5-FITC dimers and trimers (occurred also in 10 –5 mol/dm 3 concentration), which are detected in … WebFluorescein C20H12O5 CID 16850 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity …
WebFluorescein-5-maleimide is an activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) that is used to easily conjugate fluorescein to proteins. 1,2,3 The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. 4 The compound can be used to label proteins for the … WebFluorescein (FITC) Fluorescence SpectraViewer. Fluorescein derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water solubility. Fluorescein-based …
WebDescription. A fluorescent labeling agent used to label antibodies, lectins, toxins, various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9).
WebAbstract. Fluorescein (resorcinolphthalein) is a synthetic organic photoactive dye compound, first synthesized by von Bayer in 1871 [1]. Some of the advantageous features of fluorescein fluorophores are good water solubility and absorption, emission, and maximum brightness at physiological pH (Anthoni et al., 1995) [2]. eaqh002WebView Fluorescein-5-isothiocyanate (FITC) Datasheet. Toll Free: (877) 796-6397-- USA and Canada only --www.selleckchem.com. Fax: +1-832-582-8590. ... * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch ... csr plasterboard sizesWebMar 22, 2015 · The solubility data for 2,3,4,5-tetrabromothiophene dissolved in binary ethanol + trichloromethane solvent mixtures is used to describe the preferential solvation … ear0028WebApr 5, 2024 · Fluorescein isothiocyanate (FITC) was added to 20 mL of 1 mg/mL collagen carbonate buffer (0.1 M, pH 9.0), stirred at room temperature and crosslinked at 450 rpm for 18 h in the dark. The labeled collagen solution was centrifuged at 3000 rpm for 15 min, washed five times with carbonate buffer and stored at 4°C in the dark. ear1111WebApr 11, 2024 · 中文名称:硬脂酸-聚乙二醇-荧光素英文名称:Stearic acid-PEG-FITC 溶解性:溶于常规水溶液以及大多数有机溶剂稳定性:在4℃以下低温、避光,干燥12个月。 分子量(PEG ):2000、3400、5000,其他分子量可以定制。 储存条件:<4℃低温干燥保存,避光。可以在惰性气体下处理材料以获得稳定性。 ear006 formation cnam.frWebThe amine-reactive fluorescein derivatives (Amine-reactive xanthene derivatives in this section—Table 1.8) have been the most common fluorescent derivatization reagents for covalently labeling proteins.In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation … csr plasterboard sheet sizesWebInvitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. ... In contrast to the highly water soluble fluorophores ... ear001 ou ear002