WebStrengths of the Flybow approach are that it enables direct imaging of endogenous fluorescence signals in four channels and only one UAS transgene is required. Flybow-1.0 and – 1.1 constructs consist of one or two invertible cassettes, mediating the stochastic expression of two or four fluorescent proteins, respectively. WebThis chapter provides detailed technical information on how to use Drosophila variants of the mouse Brainbow-2 system, called Flybow, for stochastic labeling of cells with different fluorescent proteins in one sample. We first describe the genetic strategies and the heat shock regime required for induction of recombination events.
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Flybow to dissect circuit assembly in the Drosophila brain
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