Phis2.1 载体图谱

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August undefined, 2024

Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional … http://www.biovector.net/product/319746.html

VvWRKY30, a grape WRKY transcription factor, plays a positive ...

Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were … WebbpHis2 价格： 1000元联系方式： I47-825O-882O 立刻购买买家导航载体基本信息载体质粒图谱和多克隆位点信息载体简介载体序列 LOCUS pHIS-2 7207 bp DNA SYN DEFINITION pHIS-2 ACCESSION KEYWORDS SOURCE ORGANISM … fitch banorte

Can anyone help by suggesting a way to perform Y1H assay or …

Webb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用Y187菌株，它相对于AH109菌株在基因组上少了HIS的报告基 … Webb6 feb. 2024 · Lower panel revealed that pHis2.1 vector encompassing FL, ∆1, or ∆2 promoter region of the ANAC003 gene was amplified, respectively by using the primer set located at the pHis2.1 vector. Webb1 sep. 2000 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs (protein cloned in ... fitch baby play gym

Phis2.1 载体图谱

Webb26 maj 2024 · 质粒图谱载体描述 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was … WebbpHis2酵母表达载体说明- HaleWaihonaPukeBaidu 载体序列 ORIGIN 1 GAATTCCCGG GGAGCTCACG CGTTCGCGAA TCGATCCGCG GTCTAGAAAT ... pFLD/CAT pFLDα pGADT7-T pGAG424 pGAPZA pGAPZB pGAPZαB pGAPZαC pGBKT7 pGBKT7-53 pGBKT7-Lam pHIC-PI pHIL-D2 pHIL-S1 pHis2 pHisSi-1 pMETA pMETB pMETC pMETαA pMETαB pMETαC …

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Webb技术路线 1、文库质量评估：理论+涂板评价交付材料：反馈质量评估结果 2、载体构建：基因合成诱饵基因构建Phis2载体交付材料：含诱饵基因的质粒 3、启动子背景筛查：Binding seq基因启动子的背景筛查交付材料：阳性克隆测序结果 word项目报告 4、文库筛选：诱饵质粒转化和文库质粒转化酵母筛选和3AT条件优化筛选结果测序交付材料：阳性克隆测 … Webb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 …

WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。

Webb1 juni 2024 · Digest the pHIS2 vector with Sma I purify it by agarose gel electrophoresis (0.8% agarose). 5. For addition of a single ‘T’ base at the ends of the linearized pHIS2 plasmid, prepare the following mix in a 10 μL total volume: 1 μg pHIS2 ( Sma I linearized), 0.5 μL dTTP (stock concentration 10 mM), 1 μL 10× ExTaq buffer, 0.5 U ExTaq. http://www.biofeng.com/zaiti/jiaomu/pHis2.html

WebbVector Database. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. Only the plasmids deposited at Addgene are available for purchase through this website.

WebbpHISi载体价格3000元，pHISi载体质粒图谱(Vector map)，载体序列(Sequence)见下文。 fitch barrelsWebb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ … fitch baltimore can goodrx be used with medicaidWebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... fitch baseballWebb19 nov. 2012 · 我把要验证的转录因子构建到pGADT7-rec2载体上，与pHis2的空载共同转化Y187，本来要拿这个做阴性对照的，可是在三缺板子上长得很欢乐呀，加了3AT，并且 … can goodrx be used with medicare part dWebbpHis2酵母单杂交载体是由Biovector Co.,LTD代理或销售的Biovector, Addgene, ATCC, Invi品牌的试剂，产品来源于Biovector Inc. USA。Biovector Co.,LTD是中国最权威的pHis2酵母单杂交载体试剂销售服务商之一，在海淀区等地方销售pHis2酵母单杂交载体试剂已经多年。生物在线为您提供众多企业pHis2酵母单杂交载体仪器产品及 ... fitch baseball fields mesa azWebb16 mars 2024 · 诱饵载体：pHis2-DNA（筛选标记Trp报告基因His）文库载体：pGADT7-cDNA（筛选标记Leu）实验步骤：一、转化诱饵载体（参考SK2400中质粒转化步骤） 1. YPDA平板培养划线，活化Y187酵母菌种；挑酵母菌落于液体YPDA培养基摇菌培养； 2. 制备感受态并将pHis2-DNA转化进入感受态细胞； 3. SD/-Trp平板检测转化结果。二、自 … fitch bank