Phis2.1

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August undefined, 2024

Webb1 juni 2024 · When the insertion sequence is predicted as known cis-acting element , construct the pHIS2 reporter by three tandem copies of the studied elements inserted at … http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf

pHis2载体质粒图谱、序列、说明书、价格_in - Sohu

Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which contained … WebbpHIS2-hsSTAM1 (1-143) Sequences pHIS2-hsSTAM1 (1-143) Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Partial (1) Partial Sequences … shyann malone new show

VvWRKY30, a grape WRKY transcription factor, plays a …

WebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... Webb1 sep. 2024 · 1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes. ... and pHIS2-MiPG were cultivat ed on SD/-T r p/-His for 3 days, and . WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … shyann thomas

Transcription Factor-Centered Yeast One-Hybrid Assay

Identification and functional analysis of transcription factors …

Webb12 okt. 2024 · Moreover, a yeast one-hybrid assay was performed to verify the interaction between CnGATA20 and the CnOLE18 promoter. pHIS2.1-pro53 and pGADT7-53 (pHIS2.1-pro53+pGADT7-53) were cotransferred into yeast Y187 as a positive control, and pHIS2.1-proCnOLE18+pGADT7-53 was used as a negative control. Webb1 site: C C C A G C G G G T C G: After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. AmpR 1973 .. 2833 = 861 bp 286 amino acids = 31.6 kDa 2 segments shyann thomas bel air shyann thomas bel air high school

"Webb14 jan. 2015 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... " - Phis2.1

Phis2.1

WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates … Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1.

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Webb6 feb. 2024 · Europe PMC is an archive of life sciences journal literature. Webb16 okt. 2014 · Specifically, the bait construct pHis2.1−2 × 150 was generated by cloning tandem copies of the 150-bp enhancer (2 × 150) into the MluI site of pHis2.1, a one …

Webb27 mars 2015 · In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. As prey to select for TFs that bind to the … Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional …

Webb15 okt. 2024 · For the protein–DNA-binding experiment, an oligonucleotide sequence containing triple tandem copies of the W-box and mW-box was synthesised, annealed, and cloned into the pHIS2.1 vector forming W-box- and mW-box-specific reporter vectors. WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for …

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http://www.coolaber.com/usr/2160/File/F/F4671.pdf thepatriotscommandxsWebb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. shyano comedyWebbPlasmid pHIS2-hsSTAM1 (1-143) from Dr. James Hurley's lab contains the insert phis2- STAM1(1-143) and is published in Structure. 2009 Mar 11. 17(3):406-16. This plasmid is … the patriot party shirtsWebbyeast reporter vector phis2 1 bd ( TaKaRa ) 95. TaKaRa yeast reporter vector phis2 1 bd. Yeast Reporter Vector Phis2 1 Bd, supplied by TaKaRa, used in various techniques. Bioz … the patriot movie where to watchWebb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 … shyann on hlnWebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... shyann superstoreWebbThe promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... the patriot movie filming locations