Phospho 4ebp1 antibody
WebFigure 3: Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (AF2308) Western blot analysis of extracts from 293 cells treated with 20% FBS , using Phospho-4EBP1(Ser65/Thr70) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide. WebProduct name Anti-eIF4EBP1 (phospho T70) antibody [EPR654 (2)A] See all eIF4EBP1 primary antibodies Description Rabbit monoclonal [EPR654 (2)A] to eIF4EBP1 (phospho T70) Host species Rabbit Tested applications …
Phospho 4ebp1 antibody
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WebInvitrogen Anti-Phospho-4EBP1 (Thr37, Thr46) Polyclonal, Catalog # PA5-114544. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) (IHC (PFA)) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL). WebEach 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat.
WebThis antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1. Tested applications Suitable for: IHC-P, WB more details Species reactivity Reacts with: Rat, Human Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan Immunogen Web实验用到的供体是Thr46（铽标记的anti-phospho-4EBP1），受体是绿色荧光标记的4EBP1，是mTOR下游信号通路的蛋白，有活性的mTOR能磷酸化4EBP1，使之与供体结合。如果mTOR活性受到抑制，供体受体距离不能达到荧光能量转移的要求，则在515nm处与485nm处的信号比值增大。
WebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体， ... WebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
WebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E …
WebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ... how much lithium is in salt waterWebThe Phospho-4EBP1 (Thr37/46) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. how much lithium is in the human bodyWebMA Beigh's Publication in Biomed Res Int....Sigma unless otherwise indicated. Anti-S6K antibody (Santa Cruz Biotechnologies Inc), Anti-S6K (Polyclonal-Genscript), Anti-Erk1, Anti-phospho-Erk, Anti-4EBP1, Anti-phospho- 4EBP1-T70 and Anti-phospho-S6K Thr412 antibodies were obtained from Cell Signaling... how do i learn financeWebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes how do i learn english作文WebAnti-eIF4EBP1 (phospho T36) antibody See all eIF4EBP1 primary antibodies Description Rabbit polyclonal to eIF4EBP1 (phospho T36) Host species Rabbit Specificity ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. how much lithium is in a tesla battery packhow do i learn flasks tbcWebListed below are anti-4EBP1 antibodies from multiple suppliers. 4EBP1 is a reported alias name for the human gene EIF4EBP1, or 'eukaryotic translation initiation factor 4E binding protein 1'. ... Immunotag™ Phospho-4EBP1(Ser65/Thr70) Antibody. Applications: WB, IHC; Reactivity: Hu, Ms, Rt; Conjugate/Tag: Unconjugated, Biotin, FITC, HRP, Alexa ... how do i learn english grammar