Phospholipidosis detection reagent

WebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected … WebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is …

HCS LipidTOX™ Red Phospholipidosis Detection Reagent …

WebMar 17, 2024 · This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. This assay … WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar bodies visible under an electron microscope (EM). 1 Drugs, including many antibiotics, antidepressants, and antihistamines, induce PLD in various tissues such as lung, 2 heart, … fish oil buttock enhancement https://nevillehadfield.com

HCS LipidTOX™ Red Phospholipidosis Detection Reagent …

WebLipidTox Green neutral lipid stain and LipidTox Red phospholipidosis detection reagent (Kit from Molecular Probes) ... ORIGINAL ARTICLE 232 E-LDL and Ox-LDL Differ in the Lipid Storage. phospholipidosis detection depending on the emission spectra of the dye (Table 1) (30,37–39). Lipid-loaded macrophage foam cells play a major role in WebJan 27, 2024 · Briefly, cells were incubated in complete bronchial epithelial growth medium supplemented with 1 x phospholipidosis detection reagent for 48 hr. Cells were subsequently fixed in 4% PFA and stained with 1 X neutral lipid detected reagent for 30 min and counterstained with 1 μg mL-1 Hoechst 33258. Staining intensity was normalized to … WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar … can delirium be reversed

Invitrogen HCS LipidTOX Red Phospholipidosis Detection …

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Phospholipidosis detection reagent

Cell-Based Imaging Assay for Detection of Phospholipidosis

WebApr 1, 2015 · Phospholipidosis (PLD) is a disorder of excessive storage of phospholipids (PLs) in the lysosomes of cells and tissues. Several mechanisms have been described which may lead to the accumulation of PLs in lysosomes and can be due to genetic disorders (e.g. Niemann-Pick disease) or triggered by xenobiotics or drugs, referred to as drug-induced … WebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader … The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes

Phospholipidosis detection reagent

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WebH34157 HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit *for high content screening* *for cellular imaging* *2-plate size*. . . . . 1 kit ... H34350 HCS LipidTOX™ Green phospholipidosis detection reagent *1000X aqueous solution* *for cellular imaging* 10-plate size* . . . . . . . . . each WebHCS LipidTOX™ Phospholipidosis Detection Reagents 3 Preparing the Labeling Solution and Staining the Cells 1.1 Dilute the 1000X LipidTOX™ phospholipidosis detection …

Webdrug-induced phospholipidosis. The morphological hallmark of phospholipidosis is the appearance of unicentric or multicentric-lamellar bodies when viewed under an electron … WebNov 9, 2024 · Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological …

WebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … Webreagents containing DMSO using equipment and practices appropriate for the hazards posed by such materials. Dispose of the reagents in compliance with all pertaining local …

WebFeb 27, 2024 · The LipidTOX Green Phospholipidosis Detection Reagent is dedicated to phospholipidosis detection, which is often triggered by cationic amphiphilic drugs. Within 24 h of incubation with CQ, the increase in fluorescence intensity of LipidTOX for phospholipidosis detection was significant.

WebInvitrogen’s HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kits offer the reagents for performing image-based high-content screening (HCS) assays to … fish oil bulkWebThe HCS LipidTOX Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima approx. 595/615nm ... candelis incfish oil capsules benefits and side effectsWebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of … fish oil capsules 1200 mgWebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell … candeling ear wax hair on fireWebHCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. fish oil capsules doseWebJun 6, 2024 · The phospholipid storage was accompanied by an expansion of the endolysosomal compartment in cultured TEC as evidenced by the increased intensity of LysoTracker Red staining and the enhanced... fish oil capsule size